![]() The red appearance of these fruits results from anthocyanin accumulation 3–5. Unlike the common green stripes in watermelon, red stripes sometimes occur in apple, pear, and peach. Although such stripes form along the main carpellary vascular bundles 1, 2, the molecular mechanism of stripe formation has not been investigated. Alternating dark and light-green stripes are common in watermelon, pumpkin, and squash. Gibberellins, Secondary metabolism Introductionįruit with stripes, which generally run longitudinally, can occur naturally. We propose a fruit-striping mechanism, in which the abnormal overexpression of PbGA2ox8 in “Red Zaosu” induces the formation of a longitudinal array of anthocyanin stripes near vascular bundles in fruit. Transient overexpression of PbGA2ox8 resulting from vacuum infiltration induced anthocyanin accumulation preferentially in calcium-enriched areas near the vascular bundles in pear leaves. Moreover, the presence of red stripes indicated anthocyanin accumulation in the hypanthial epidermal layer near vascular branches (VBs) in “Red Zaosu”. ![]() Transient overexpression of PbGA2ox8 reduced the GA 4 level and caused anthocyanin accumulation in pear fruit skin. Moreover, the GA levels in “Red Zaosu” seedlings were lower than those in “Zaosu” seedlings, and the application of exogenous GA 4 reduced abnormal anthocyanin accumulation in “Red Zaosu”. Consistently, decreased methylation was also observed in the promoter region of PbGA2ox8 from “Red Zaosu” compared with “Zaosu”. In this study, comparative transcriptome analysis of the sport and wild-type “Zaosu” revealed that this atypical anthocyanin accumulation was tightly correlated with abnormal overexpression of the gene-encoding gibberellin (GA) 2-beta-dioxygenase 8, PbGA2ox8. Previously, we observed an atypical anthocyanin distribution that caused red-striped fruit on the spontaneous pear bud sport “Red Zaosu” ( Pyrus bretschneideri Rehd.). Its post merger total is 9,227.Fruit with stripes, which are generally longitudinal, can occur naturally, but the bioprocesses underlying this phenomenon are unclear. 29, Laval-based Couche-Tard's network consisted of 8,081 convenience stores throughout North America. That deal is still waiting for regulatory approval and hasn't been completed yet. ![]() CST's 1,146 stores operated under the Valero Corner Store, Nice N Easy and Flash Foods banners. is being acquired for $4.4 billion by Canada-based Alimentation Couche-Tard, the parent company of Circle K. All the international licensing in 17 countries, with the exception of Japan, is handled by 7-Eleven in Irving. Worldwide 2016 sales exceeded $89 billion. and Canada sales were estimated at $25 billion, with 60 percent or $15 billion coming from inside the store and 40 percent from gasoline pumps, according to a recently published list of top food retailers from Supermarket News. This acquisition will bring 7-Eleven's total number of stores to 9,815 in the U.S.
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